Analysis of manual reticulocyte counting

Manual reticulocyte counts were examined under light microscopy, using the property whereby supravital stain precipitates residual ribosomal RNA versus the automated flow methods, with the Estimated Reading Time: 6 mins. Peripheral blood reticulocyte enumeration is a rou- tine assay performed by counting dried smears of blood previously stained with RNA-precipitating dyes (4, 13, 22). Serial reticulocyte counts are a sensitive indicator of erythropoiesis (); however, the assay is impre- cise and inaccurate (22). The sources of error are fromCited by: Abstract. A statistical appraisal of manual reticulocyte enumeration was extensively investigated. Statistically, the counting variability among technologists was significantly worse than expected for a counting (Poisson) process. the proportional error associated with each technologist can exceed 39%.Cited by:

Abstract. Reticulocyte counting by flow cytometry with thiazole orange was compared to manual or automated counting of new methylene blue stained blood smears. Forty‐nine samples were compared for manual counting from randomly chosen clinical samples. Two hundred and eighty‐nine samples from bone marrow transplant patients were compared during the period before and through chemo‐irradiation and engraftment. Abstract. A statistical appraisal of manual reticulocyte enumeration was extensively investigated. Statistically, the counting variability among technologists was significantly worse than expected for a counting (Poisson) process. the proportional error associated with each technologist can exceed 39%. A manual reticulocyte count is performed in the event that the automated reticulocyte count is beyond the reportable range of the analyzer, the sample is insufficient to perform by automated methodology or the sample is obtained from a patient with a Hemoglobinopathy. At no time are the manual and automated result reported for the same sample. A. Staining 1.

Apr The manual reticulocyte counting by microscopy became traditional and hasbeen considered the standard method since , for its simplicity and. Oct a clinical analysis laboratory, where the BD FACSCalibur flow Each sample underwent two manual reticulocyte counts. 5 days ago Pathophysiology · Reticulocyte count determines: Bone marrow function. · The reticulocytes production index (RPI) corrects the hematocrit to a.